Puc18 cloning vector
WebpUC18 vector. 2. pUC plasmids: • pUC plasmids are small, high copy number plasmids of size 2686bp. • This series of cloning vectors were developed by Messing and co-workers … WebA small backbone also opens up for additional unique restriction enzyme cloning sites. Here we describe the generation of pICOz, a 1185-bp fully functional high-copy cloning plasmid …
Puc18 cloning vector
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WebRestriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. Many restriction enzymes make staggered cuts, producing ends with … http://www.sfc.ac.in/pdf/syllabus/sciences/biotech/Biotech/Molecular_Biology_rDNA%20_Technology_T_SEM%203.pdf
WebUSD $67.00. pHSG298 is a kanamycin-resistant pUC cloning vector which contains the same consensus multicloning site as pUC18 (though Hind III and Sma I sites are not available). Using this vector, it is possible to transfer a DNA fragment from pUC19 to pUC18 without the preparation of DNA fragments. Notice to purchaser. WebProduct of the synthesis was cloned into the auxilliary cloning vector pUC19. ... Construct will be further used as an expression vector to produce of the variants Var 14 GCPII in eucaryotic baculovirus expression system. Keywords: 5'UTR, …
WebVector Backbone pUC19. Addgene. Addgene: pUC19-TSV (linearized in LT) EnzyQuest. pUC19 DNA plasmid - EnzyQuest. Chegg. Solved The extracted plasmid DNA is pUC19. Look up the Chegg.com. A General Biology and Molecular & Cell Biology Resource … WebMar 10, 2024 · A cloning vector is a small piece of DNA into which a piece of foreign DNA is inserted for cloning. A vector could be a DNA molecule that prevents foreign DNA from entering the host cell. It has the ability to self-replicate and integrate into the host cell. A cloning vector receives target DNA and then replicate it autonomously, increasing the ...
Web4、gle-Stranded Phage VectorM13,f1,fdM13 PhageIII.Single-Stranded Phage Vector（1）+DNA。(ssDNA)（2）The replication type(RF)is double-stranded circular DNA.（3）Both RF DNA and ssDNA can transfect competent E.coli.And produce plaque.（4）There are no packaging restrictions.（5）The phage can produce large quanti.
WebDec 23, 2013 · The pUC18/Kan reporter vector was prepared using PIPE cloning. The vector backbone of pUC18 was obtained by PCR with primers pUC18–F and pUC18–R, and the kanamycin resistance cassette from pEGFP-C1 (Clontech) with … find used car onlineWebpUC18 is a small, high copy cloning vector for replication in E. coli.It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322.The … find used car parts salvage yards near meWebDec 11, 2024 · 2.2 Genomic DNA extraction and construction of vectors The CTAB DNA extraction protocol was used to extract genomic DNA [14]. The pUC19 plasmid was modified into a gateway compatible entry vector pUC19. In short, the gDNA sequence was amplified from plant genomic DNA and transferred into the entry vector pUC19 using one … erin eagles therapistWebDescription. pUC18 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 … find used cars areaWebA 5-kb EcoRV fragment of this insert was subcloned in grown to late log phase at 37°C in Trypticase soy broth, harvested by centrifu- the SmaI site of cloning vector pUC18 to construct the plasmid pUCAB, which gation, washed once with 50 mM sodium phosphate buffer (pH 7.0), resuspended was then sequenced. find used car progressiveWebThe pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector). These cloning vectors contain a … erin easingwoodhttp://www.biovector.net/product/1266904.html erin earth presentation sisters