WebNov 29, 2012 · In the present study, CSFV Shimen strain full-length genomic cDNA carrying a genetic tag (AvaI site) was cloned downstream of the cytomegalovirus (CMV) … WebNov 29, 2012 · In the present study, CSFV Shimen strain full-length genomic cDNA carrying a genetic tag (AvaI site) was cloned downstream of the cytomegalovirus (CMV) promoter and T7 promoter to enable rescue by either of the methods described above. To ensure integral and precise transcription of the genome, the genomic cDNA was flanked …
Characterization of the rescued CSFVs. (A) Growth kinetics of the...
WebClassical swine fever (CSF) caused by the classical swine fever virus (CSFV) has resulted in severe losses to the pig industry worldwide. It has been proposed that lipid synthesis is essential for viral replication, and lipids are involved in viral protein maturation and envelope production. However, the specific crosstalk between CSFV and host cell lipid metabolism … WebNov 19, 2024 · Then, the cells were stimulated with ultraviolet-light-inactivated CSFV (Shimen strain; MOI = 1) l. After 24 h of incubation at 37 °C, the cell-free supernatant was removed and detected using a commercially available porcine IL-4 and IFN-γ ELISA kit according to the manufacturer’s protocol. OD values were measured at 450 nm using a ... fish you can catch in obx
Pesquisa Portal Regional da BVS
WebDec 1, 2013 · The strains of CSFV (Shimen and C) used were obtained from the Control Institute of Veterinary Bioproducts and Pharmaceuticals (China). Virus strains were … WebJul 22, 2024 · The CSFV Shimen strain (GenBank accession number AF092248.2) was generated from a cDNA clone (pSPT I /SM) and propagated in PK15 cells. The BVDV I Hubei strain (GenBank accession number MZ484396) is a passaged variant of BVDV I NDAL strain (GenBank accession number AJ133738.1) [ 19 ] and propagated in MDBK … WebMay 1, 2024 · The chimeric CSFV/BVDV fragment consisted of the E2 gene of BVDV and p7 gene and incomplete NS2–3 coding region of CSFV, which was obtained from overlap PCR amplification using the cDNAs of BVDV Hubei strain and CSFV Shimen strain as templates. The pCSFV/bE2 mutants were constructed using genetic approaches based … fish you can catch in march animal crossing